A Tutorial and Hands-on Workshop on High-Throughput Sequencing: Analytics & Bioinformatic Tools and Programs

Event Date 

May 19, 8:00am to 5:00pm


Institute for Food Safety and Health
6502 S. Archer Road
Bedford Park, IL 60501



High-Throughput Sequencing (HTS) is becoming the new gold standard in many fields including food safety. The governmental agencies that monitor or regulate the food industry and human health, both domestically and internationally, have taken major steps in adopting and implementing it in their routine investigative and regulatory work. While there is a growing interest among the food companies towards this new technology, its practical use and implementation to ensure food safety has been limited at best, partly, because the technology is still relatively new; its uses by the industry demand certain level of proficiency; the HTS data are large; the analytical work is intricate and complicated; and the results seem hard to interpret.
To address these issues, the IFSH HTS Initiative (IHI) will present a series of tutorials and workshops customized, specifically and mainly, for food safety officers and technicians in the food industry, as well as the university students who study food safety and science. The first in this series is a joint IFSH & FDA tutorial and workshop that presents a gateway into the realm of HTS technology, data analyses and bioinformatics. While this is an entry level tutorial and workshop, participants with more extensive knowledge and experience will certainly benefit from its interactive nature.


The participants will learn:
►  The underlying terminology and concepts
►  Brief history and advances in DNA/RNA sequencing technology and various platforms
►  HTS applications
  • Whole Genome Sequencing (WGS)
  • Metagenomics
  • RNA-Seq/transcriptomics
►  Sequence data, data format, data handling and manipulations
►  Tools and programs commonly used in the analyses of HTS data
  • Brief introduction to terminal and command lines
  • Sequence quality control and trimming
  • Sequence assembly
  • Gene and genome annotation
  • Genome visualization
►  Phylogenetic & phylogenomics
►  Interpretation of results
Participants are required to bring their own laptops. There is no other prerequisite for this training and workshop. It will be a full day event, from 8:00 am to 5:00 pm. A continental breakfast, lunch, snacks and coffee will be provided.
High-performance computers (HPCs) of IHI will be used to demonstrate fast and real time analysis of the sample data. Students will be able to access IHI’s HPCs through a temporary account on these servers.
Space will be limited to 30 attendees, and a certificate of completion will be awarded to the participants at the end of the training session.
Participants are also encouraged to attend the IFSH HTS 2-day symposium on May 20 – 21, 2020, held  at Chicago Marriott Southwest, Burr Ridge, Illinois.


Eric L. Stevens, Ph.D.
Staff Fellow, Office of Regulatory Science
Food and Drug Administration
Renmao Tian, Ph.D.
Research Scientist, Microgenomics
Institute for Food Safety and Health
Illinois Institute of Technology
Jean-Francois Pombert, Ph.D.
Associate Professor of Biology
Illinois Institute of Technology
Academic Editor, PLOS ONE
Behzad Imanian, Ph.D.
Research Assistant Professor
Leader, IFSH HTS Initiative
Institute for Food Safety and Health
Illinois Institute of Technology

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