High-Throughput Sequencing Initiative Research Projects


IFSH High Throughput Sequencing Topics of Investigation

  • Foodborne bacterial community composition and its transitional stages in the cycle of production, processing, packaging, storage, distribution and consumption of different foods
  • Mapping the biogeography of foodborne bacterial communities in crop and animal farms
  • Human gut microbiome and its changes in response to the dietary regimes of interest
  • Toxin production and regulation in model bacteria including Clostridium botulinum
  • Rapid and reliable identification of foodborne microorganisms including bacteria, viruses, and eukaryotic microbes of interest
  • Bacterial resistance against antibiotics, sanitizers, disinfectants, heat, cold and pressure
  • Evolution of pathogenicity in foodborne bacteria

IFSH High Throughput Sequencing Research Projects

  • Investigating the possible mutagenic effects of manipulating the treatment time, frequency, energy and voltage on a Pulsed Light instrument in the genome of Listeria monocytogenes
  • Evaluating the use of Whole Genome Sequencing (WGS) in linking contaminated seed to sprout in recent Salmonella outbreaks
  • Characterization of more than 100 Group I and Group II Clostridium botulinum strains with a focus on toxin gene clusters
  • Identifying the genes involved in the regulation of botulinum neurotoxin production, using transcriptomics (RNA-seq) of Clostridium botulinum, investigating the role of growth media, pH and temperature
  • Investigating microbial community composition and characterization of transitional stages of this community in conjunction with the biofilm formation in an industrial setting
  • Investigating the effects of select sanitizers on the structure and composition of microbial community in various industrial and laboratory settings
  • Characterization and comparison of soil microbial communities in Detroit city community gardens 

HTS Services And Facilities


HTS Symposiums And Presentation Materials

The IHI is also active in educational events and training programs. Since its start in 2016, the IHI has organized the following HTS symposiums:

              A Tutorial and Hands-on Workshop to Help Understand Its Application to Food Safety (May 6-7, 2019)

Key Staff Members And Contact Information 

Behzad Imanian, Ph.D.

Research Assistant Professor, Leader of IFSH High Throughput Sequencing, IIT


Research Scientist Microbial Genomics, IFSH High-Throughput Sequencing, IIT

Membership Application Process

RENEE mcbrien