Allergens are a growing concern for both consumers as well as food manufactures as the prevalence of food allergies rises in the population. Biotechnology and the application of immunological based assays has helped to elucidate the challenges of producing allergen free products. However, the reliability of the data from these techniques is sometimes confounded by the matrix and/or the process through which the allergen passes. Moreover these typically target only one allergen at a time. Consequently, the chemistry team at IFSH in collaboration with its members and the FDA has been working to develop of mass spectral based multiplexed assay as an alternate method of detecting allergens in complex food matrices.